Haploid cells of the yeast Saccharomyces cerevisiae express either an a- or α mating-type. The respective cells secrete small peptides as pheromones, which is called α-factor in the case of α-cells. This pheromone binds to specific receptors of cells of the opposite mating type, inducing the pheromone signal cascade and a genetic program, leading to e.g. morphological changes - the so called "Shmoo" - and a cell-cycle arrest.
For heterologous gene expression using e.g. promotors of genes of the mating response pathway as well as for "Shmoo" induction or cell-cycle arrest we recommend the use of 10 - 100 µM, depending on the yeast host strain.
Datasheet Alpha - Factor - HG-AF-0001